|
KMID : 0364020170500020126
|
|
Korean Journal of Thoracic and Cardiovascular Surgery
2017 Volume.50 No. 2 p.126 ~ p.129
|
|
|
Circulating Aneuploid Cells Detected in the Blood of Patients with Infectious Lung Diseases
|
|
Kim Hong-Sun
Cho Jong-Ho
Sonn Chung-Hee
Kim Jae-Won
Choi Yul
Lee Jin-Seon
|
|
|
Abstract
|
|
|
|
The identification of circulating tumor cells (CTCs) is clinically important for diagnosing cancer. We have previously developed a size-based filtration platform followed by epithelial cell adhesion molecule immunofluorescence staining for detecting CTCs. To characterize CTCs independently of cell surface protein expression, we incorporated a chromosomal fluorescence in situ hybridization (FISH) assay to detect abnormal copy numbers of chromosomes in cells collected from peripheral blood samples by the size-based filtration platform. Aneuploid cells were detected in the peripheral blood of patients with lung cancer. Unexpectedly, aneuploid cells were also detected in the control group, which consisted of peripheral blood samples from patients with benign lung diseases, such as empyema necessitatis and non-tuberculous mycobacterial lung disease. These findings suggest that chromosomal abnormalities are observed not only in tumor cells, but also in benign infectious diseases. Thus, our findings present new considerations and bring into light the possibility of false positives when using FISH for cancer diagnosis.
|
|
|
KEYWORD
|
|
|
Circulating neoplastic cells, Fluorescent in situ hybridization, Lung neoplasms
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|